Journal: Frontiers in Cell and Developmental Biology

Article Title: Elucidating the Biomechanics of Leukocyte Transendothelial Migration by Quantitative Imaging

doi: 10.3389/fcell.2021.635263

Figure Lengend Snippet: Summary of cellular force measurement techniques.

Article Snippet: Molecular tension-fluorescence lifetime imaging microscopy (MT-FLIM) , Force threshold F 1/2 is a measure of the applied force at which 50% of probes are open, estimating applied force ranges; Force resolution to the order of pN exerted by individual integrins , Supported lipid bilayer (SLB) – phospholipid membranes; Confined in the Z-direction but are laterally fluid , Inverted microscopes with perfect focus capabilities and appropriate lasers for excitation; Specific software required; , Matlab Bioformats Toolbox and semiautomated custom scripts; FIJI plugins, including MultiKymograph and TrackMate , Highly specific observations of integrin behavior and force generation as it relates to podosome formation and mechanosensing , Highly technical in the development and implementation of molecular tension probes, and in microscopy set-up Misses forces transmitted via non-specific interactions , ; ; ; ; .

Techniques: Microscopy, Confocal Microscopy, Single-particle Tracking, High Throughput Screening Assay, Fluorescence, Imaging, Modification, Sample Prep, Membrane, Software, Comparison